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Soluble in water; slightly soluble in ethanol, methanol.
Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for the staining of blood films. It is also used for the localization of nucleic acids.Azure B has been used for the staining of chromosomes. Azure B has been used in M'Fadyean stain to visualize Bacillus anthracis capsule.
Azure B is used for differentiating cellular RNA (purplish) and DNA (blue-green) in plant tissues. Useful as a stain for Negri Bodies. Can be used in the Romanowsky-type stains for blood and blood protozoa. Purified azure B, on the other hand, gave reproducibly stained, deposit-free preparations. Reticulocyte counts obtained from azure B preparations correlated almost exactly with those determined using new methylene blue. Purified azure B is therefore recommended as a convenient reticulocyte stain for routine use.