GSP Chemical Industries

Nile Blue Chloride

Nile Blue Chloride
Business Type Manufacturer, Supplier
C.A.S. No. 2381-85-3
CI NO. 51180
M.F. C20H20ClN3O
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Preferred Buyer From

Location Anywhere in India

Product Details

Dye Content (by Spectrophotometry)
Solubility 0.1% (50% EtOH)
Clear solution
Absorption maximum (50% EtOH) ?max
Absorptivity (A1%/1cm in 50% EtOH, at ?max)
Loss On Drying (110°C)

Chemical name

Bis(5-amino-9-(diethylamino)benzo(a)phenoxazin-7-ium) chloride


InChI Key




Soluble in ethyl alcohol


Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies. Biochem/physiol Actions. Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0).



Nile Blue chloride, or Nile Blue A, is a fluorescence reference standard used for various histologic staining. It is commonly utilized in studies involved with lipid and nuclei staining. Nile Blue chloride imparts a pink color to unsaturated glycerides and neutral lipids and stains nuclei and acids (such as fatty acids, chormolipids and phospholipids) a purple-blue color.

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