Nile Blue Chloride

Chemical name

Bis(5-amino-9-(diethylamino)benzo(a)phenoxazin-7-ium) chloride

InChI Key

SHXOKQKTZJXHHR-UHFFFAOYSA-N

Solubility

Soluble in ethyl alcohol

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies. Biochem/physiol Actions. Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0).

Applications:

Nile Blue chloride, or Nile Blue A, is a fluorescence reference standard used for various histologic staining. It is commonly utilized in studies involved with lipid and nuclei staining. Nile Blue chloride imparts a pink color to unsaturated glycerides and neutral lipids and stains nuclei and acids (such as fatty acids, chormolipids and phospholipids) a purple-blue color.