GSP Chemical Industries

Bromocresol Green ACS Grade

Bromocresol Green ACS Grade
Business Type Manufacturer, Supplier
C.A.S. No. 76-60-8
M.F. C21H14Br4O5S
M.W. 698.04gm/mole
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Preferred Buyer From

Location Anywhere in India

Product Details

Dye Content (by Spectrophotometry)
Transition Range
pH 3.8-5.4 Greenish Yellow-Blue
Solubility 0.1% (95% EtOH)
Clear solution
Absorption maximum (pH 3.8) ?1max
Absorption maximum (pH 5.4) ?2max
Absorptivity (A1%/1cm at pH 3.8 at ?1max)
Absorptivity (A1%/1cm at pH 5.4 at ?2max)
Loss On Drying (110°C)

Chemical Name

3,3-Bis(3,5- dibromo-4- hydroxy-2- methylphenyl)-

3H-benzo[c][1,2]oxathiole 1,1-dioxide


InChI Key



Bromocresol Green is a dye of the triphenylmethane family (triarylmethane dyes). It binds quantitatively with human albumin forming an intense blue-green complex with an absorbance maximum at 628 nm.


Format Powder

pH 3.8 - 5.4, yellow to blue(Lit.)



Soluble in water (6 mg/mL), ethanol (40 mg/mL) or methyl cellosolve (200 mg/mL).


Storage and Handling: Store at Room Temperature(15- 30 °C).


Density : ~2.1 g/cm 3 (Predicted)


pK a : 4.24 (Predicted)



Bromocresol Green is useful in a variety of colorimetric detection technologies. It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge transfer complexation processes. In TLC, it is used for visualisation of the compounds with functional groups whose pKa is below 5.0. Bromocresol green is a pH indicator and reagent. It has been used in serum albumin determinations and as a pH indicator.It is a pH sensitive triphenylmethane dye useful in various colorimetric detection studies and as an activity tracking dye for DNA agarose gel electrophoresis. It is also used in TLC (thin-layer chromatography) for visualizing compounds containing functional groups having pKa less than 5.

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